Research Alert

Article title: Real-time visualization of titin dynamics reveals extensive reversible photobleaching in human induced pluripotent stem cell-derived cardiomyocytes

Authors: Adrian G. Cadar, Tromondae K. Feaster, Kevin R. Bersell, Lili Wang, TingTing Hong, Joseph A. Balsamo, Zhentao Zhang, Young Wook Chun, Young-Jae Nam, Michael Gotthardt, Björn C. Knollmann, Dan M. Roden, Chee C. Lim, Charles C. Hong

From the authors: "Our results reveal that reversible photobleaching accounts for the majority of titin recovery in the titin-mEos3.2 hiPSC-CM model and should warrant as a caution in the extrapolation of reliable FRAP data from specific fluorescent proteins in long-term cell imaging.” 

This study is highlighted as one of January's "best of the best" as part of the American Physiological Society's APSselect program.


Journal Link: American Journal of Physiology - Cell Physiology